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KMID : 0385520150280010065
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Analytical Science & Technology
2015 Volume.28 No. 1 p.65 ~ p.71
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Development of intracellular organelle markers using modified glycolipid-binding peptides in mammalian cells
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Jun Yong-Woo
Jang Deok-Jin
Lee Jin-A
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Abstract
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Intracellular organelles in eukaryotic cells play important roles in many cellular functions. Intracellular trafficking of many proteins to specific intracellular organelles is tightly regulated by various mechanisms in cells. Therefore, elucidating the targeting mechanism of novel markers for intracellular organelles is important for cellular physiology and pathology. In this study, we tried to identify the peptides which could bind to specific glycolipid in cellular membrane using GFP-fused glycolipid-binding peptides, and analyzed their cellular localization. As a result, we could identify mitochondria-, Golgi- or plasma membrane-targeting peptides. Furthermore, we found that the plasma membrane-targeting peptide was localized to the plasma membrane via electrostatic interactions. Thus, our results suggest that various glycolipid-binding peptides could be used as intracellular organelles markers.
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KEYWORD
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glycolipid-binding peptide, intracellular organelles, marker, plasma membrane, electrostatic interaction,
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